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KMID : 0356620160310040586
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Journal of Korean Society of Endocrinology
2016 Volume.31 No. 4 p.586 ~ p.591
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Molecular Diagnosis Using Residual Liquid-Based Cytology Materials for Patients with Nondiagnostic or Indeterminate Thyroid Nodules
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Kwon Hye-Mi
Kim Won-Gu
Eszlinger Markus
Paschke Ralf
Song Dong-Eun
Kim Mi-Jin
Park Su-Yeon
Jeon Min-Ji
Kim Tae-Yong
Shong Young-Kee
Kim Won-Bae
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Abstract
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Background: Molecular analysis for common somatic mutations in thyroid cancer can improve diagnostic accuracy of fine-needle aspiration cytology (FNAC) in the nondiagnostic or indeterminate category of thyroid nodules. In this study, we evaluated the feasibility of molecular diagnosis from residual liquid-based cytology (LBC) material after cytological diagnosis.
Methods: This prospective study enrolled 53 patients with thyroid nodules diagnosed as nondiagnostic, atypia of undetermined significance (AUS), or follicular lesion of undetermined significance (FLUS) after FNAC. DNAs and RNAs were isolated from residual LBC materials. BRAFV600E and RAS point mutations, PAX8/peroxisome proliferator-activated receptor γ (PPARγ), RET/PTC1, and RET/PTC3 rearrangements were evaluated by real-time polymerase chain reaction and pyrosequencing.
Results: All DNAs from 53 residual LBC samples could be analysed and point mutations were detected in 10 samples (19%). In 17 AUS nodules, seven samples (41%) had point mutations including BRAF (n=4), NRAS (n=2), and KRAS (n=1). In 20 FLUS nodules, three samples (15%) had NRAS point mutations. RNA from only one FLUS nodule could be analysed for rearrangements and there was no abnormality.
Conclusion: Molecular analysis for BRAF and RAS mutations was feasible in residual LBC materials and might be useful for diagnosis of indeterminate thyroid nodules.
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KEYWORD
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Molecular diagnostic techniques, Biopsy, fine-needle, Thyroid aspiration, Thyroid nodule, Thyroid neoplasms
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